FIGURE

Fig. 3.

ID

ZDB-FIG-211025-103

Publication

Robinson et al., 2021 - Flow cytometry allows rapid detection of protein aggregates in cellular and zebrafish models of spinocerebellar ataxia 3

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3.

Treatment with autophagy pathway modulators altered the number of EGFP-fused particles present in cells expressing human ataxin-3 with 84 glutamines. (A) Representative scatterplots reveal a scarcity of EGFP-fused particles in untransfected control cells and altered numbers of particles following treatment with autophagy modulators (3MA and calpeptin). (B) Quantification of the number of DAPI⁺ nuclei revealed that a similar number of nuclei were present across vehicle-treated and 3MA-treated samples. (C) Comparison of cells treated with vehicle or 3MA (5 mM) revealed a statistically significant increase in detergent-insoluble particles relative to the number of nuclei following 3MA treatment. (D) Similar numbers of nuclei were quantified in samples treated with a vehicle control or calpeptin. (E) Calpeptin treatment produced reductions in detergent-insoluble particles, as detected by FloIT. Data are mean�s.e.m. n=2-6 experimental replicates. *P<0.05, **P<0.01 (one-way ANOVA with Tukey's post-hoc test or Student's t-test, paired, two-tailed). AU, arbitrary units; RFU, relative fluorescence units.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.