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Expression analysis of apoptosis-related genes in bid-overexpressed embryos without (A–C) or with (D–F)E. ictaluri infection. The fertilized eggs were microinjected with 200 ng/μl p3×FLAG empty plasmid or Bid-FLAG plasmid. The microinjected eggs were raised to 4 dpf, and the expressions of apoptosis-related genes were examined using qPCR. The 4-dpf microinjected larvae were infected with E. ictaluri, and the expressions of apoptosis-related genes were examined using qPCR at 12 hpi. The expression levels of target genes were analyzed using the 2-ΔΔCT method by respectively calculating with the Ct values of gapdh(A, D), EF-1α(B, E), and the average Ct of gapdh and EF-1α(C, F). The average Ct values of the two reference genes are listed in Table S2. *p < 0.05, **p < 0.01. ns, no significance.
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