FIGURE

Figure 5

ID

ZDB-FIG-210915-60

Publication

Hong et al., 2021 - Zebrafish Bioassay for Screening Therapeutic Candidates Based on Melanotrophic Activity

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Figure 5

Melanophore restriction array assay comparing human α-MSH and MT-II. (a) Diagram representing the two-dimensional structure of α-MSH and MT-II. (b) Image of ZEBRA (zebrafish restriction array) device with major structural features labeled. The gray box indicates the region of magnification at imaging steps. (c) Protocol of melanophore restriction array assay. Red asterisks indicate imaging timepoints, while blue asterisks indicate timepoints for washing. (d) Curve shows the change of mean melanophore area over the treatment time for each peptide and concentration. Each curve aggregates the average of three trials (n = 40–50 melanophores from 4–5 larvae for each trial). Data represents mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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