FIGURE

Figure 3

ID

ZDB-FIG-210902-79

Publication

Lu et al., 2021 - Lipopolysaccharides Enhance Epithelial Hyperplasia and Tubular Adenoma in Intestine-Specific Expression of kras^V¹² in Transgenic Zebrafish

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Figure 3

Expression of kras^V¹² decreased goblet cell number, goblet cell size, villi height, and intervilli space and increased villi width and smooth muscle thickness in the intestine. At 4 mpf, WT and kras+ zebrafish were treated with 2 μM of mifepristone and 40 ng/mL of LPS for 4 weeks, and samples were then collected for gross observations and histological analysis. There were four experimental groups: WT, WT/LPS, kras+, and kras+/LPS (WT, N = 20; WT/LPS, N = 20; kras+, N = 20; kras+/LPS, N = 20). (A) PAS staining was carried out in intestinal sections. (B–G) Quantification of goblet cell number, goblet cell size of villi, villi height, intervilli space, villi width, and smooth muscle thickness in the intestine. Differences among the variables were assessed using Student’s t-tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT gz37Tg
Conditions:	chemical treatment by environment: lipopolysaccharide chemical treatment by environment: lipopolysaccharide chemical treatment by environment: mifepristone
Observed In:	intestinal villus intestine goblet cell intestine smooth muscle
Stage:	Adult

Phenotype Detail

Acknowledgments

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