Figure 4
- ID
- ZDB-FIG-210902-293
- Publication
- Pinto et al., 2021 - Zebrafish Motile Cilia as a Model for Primary Ciliary Dyskinesia
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The zebrafish OP has two different types of cilia. (A) Schematic representation of 5 dpf zebrafish head, structures of interest are marked with arrows—olfactory pits (OP). (B) TEM low magnification image showing a cross-section across an OP of a WT zebrafish, a bowl-shaped structure containing multiciliated cells and cilia in several orientations. In the periphery of this pit (dotted boxes a and inset), cilia containing classical motile structure 9 + 2 with dynein arms (black arrowheads). In the most internal region of the OP (dotted box b and inset) we detected cilia with 9 + 2 ultrastructural arrangement without dynein arms (red arrowheads); this pattern was visible in WT embryos (n = 3). (C) Snapshot from a movie of beating OP cilia and respective heatmap by software CiliarMove (C’), highlighting a region of immotile cilia (arrowhead) that was coincident with the region detected by TEM in B. n—nucleus; cb—cilia border; OP—olfactory pit. Thin bar 6 µm, thick bar 100 nm. |