FIGURE

Figure 3

ID

ZDB-FIG-210827-5

Publication

Sitaraman et al., 2021 - Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons

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Figure 3

Knocking out Gjd2b results in decrease of glutamatergic synaptic number.

(A) Representative miniature excitatory postsynaptic current (mEPSC) recordings from Purkinje neurons (PNs) of wild-type (black trace) and gjd2b^-/- (red) larvae. (B) Average mEPSC shown on expanded time base recorded from wild-type (black) and gjd2b^-/- (red) larvae. Neurons were held at −65 mV. Inset: scaled mEPSC to show faster rise time and decay time of mEPSCs in mutants. (C–F) Cumulative probability histograms and boxplots reveal increased inter-event intervals (C), increased peak amplitudes (D), decreased 10–90% rise times (E), and decreased decay time constants (F) of mEPSCs in gjd2b^-/- larvae (red lines) compared to wild type (black lines). N = 8 cells in wild type and 10 cells in gjd2b^-/- larvae. *p<0.05; ****p<0.0001; Mann–Whitney U test. Data used for quantitative analyses are available in Figure 3—source data 1. See also Figure 3—figure supplement 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gjd2b^{ncb215/ncb215} (IND)
Observed In:	Purkinje cell action potential initiation Purkinje cell chemical synaptic transmission
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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