Figure 2

The lower incidence of activated NSCs close to aNPs is Notch signaling dependent

(A) Confocal whole-mount view of the pallial germinal layer in a 3mpf Tg(gfap:tdTomato);Tg(delta:GFP) fish stained for tdTomato (NSCs, green), GFP (deltaA expression, cyan) and PCNA (magenta) (DeltaA channel only). Anterior, left. (A) Stitch of 4 tiles with 15% overlap.

(B–B’’’) Close-ups in Dm, all channels. Arrowheads: magenta, aNSC; orange, aNP; expressing deltaA.

(C–C’’) Percentages of aNSCs (all, pre-, or post-division) among all NSCs (C and C”) or aNSCs (C’) upon 24-h LY treatment (DMSO: control). ns, non-significant; unpaired t test (see also Figures S2F, S3A–S3C, and S4A–S4B’’).

(D–D”) Number (D and D’) and proportion (D’’) of aNSCs, in total (D) or touching aNPs upon 24-h LY treatment (DMSO: control) (D’’, p = 0.002, t test) (see also Figure S3C).

(E) Spatial distribution (Besag’s L function) of aNSC singlets relative to each other (left) and to aNPs (right) upon 24-h LY treatment (DMSO: control). L_(obs)(r) (red lines): experimental values; L_(H0,mean)(r) (black dotted lines): means under the random labeling null hypothesis; r: mean NSC diameter; gray regions: 95% confidence envelopes. Arrowheads: red, aNP inhibitory effect; black, disappearance upon LY (see also Figures S4C–S4F).

Scale: (A) 40 μm, (B)–(B’’’) 20 μm.