FIGURE

FIGURE 2

ID

ZDB-FIG-210822-17

Publication

Myllymäki et al., 2021 - Metabolic Alterations in Preneoplastic Development Revealed by Untargeted Metabolomic Analysis

Other Figures

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FIGURE 2

HRAS^G12V expressing PNCs show increased proliferation and altered morphology from 24 hpi. (A) Representative confocal image showing EdU labeling (Magenta) in skin cells expressing eGFPCAAX (Green) at 24 hpi. (B) Representative confocal image showing EdU labeling (Magenta) in skin cells expressing eGFPHRAS^G12V(Green) at 24 hpi. Yellow arrows indicate EdU^+ve eGFP^+ve cells, scale bar = 20 μm. (C) Graph showing increased proliferation in PNCs compared to eGFPCAAX expressing healthy skin cells 12 hpi (p = 0.057, n = 10); 24 hpi (p = 3.602e-011, n = 10), 36 hpi (p = 1,021e-07, n = 10), unpaired t-test with Holm-Sidak method for correction of multiple comparisons. (D) Assessing cell morphology shows that from 24 hpi, a significant proportion of PNCs have lost the normal polygonal shape and become elongated or developed long filopodia (one-way ANOVA with Tukey’s multiple comparisons test, ****p < 0.0001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ed201Tg; ed203Tg
Condition:	chemical treatment by environment: tamoxifen
Observed In:	integument cell integument cell population proliferation
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol