FIGURE

FIGURE 7

ID

ZDB-FIG-210729-30

Publication

Tang et al., 2021 - Inhibitory Role of an Aeromonas hydrophila TIR Domain Effector in Antibacterial Immunity by Targeting TLR Signaling Complexes in Zebrafish

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FIGURE 7

Examination of the functional role of TcpAh in preventing TRIF–TRAF3 and TRAF3–TBK1 interactions. (A,B) Activation of human IRF3 promoter in HEK293T cells transfected with human IRF3 luciferase reporter (HsIRF3-Luc; 200 ng/mL), renilla luciferase reporter (15 ng/mL), and (A) zebrafish TRAF3 expression vector (50 ng/mL) alone or in combination with zebrafish TRIF expression vector (50 ng/mL) with or without TcpAh expression vector (500 ng/mL) or (B) zebrafish TBK1 expression vector (50 ng/mL) alone or in combination with a zebrafish TRAF3 expression vector (50 ng/mL) with or without TcpAh expression vector (500 ng/mL). Data are the average luciferase activity ± SD (*p < 0.05; **p < 0.01; ns, not significant). (C–E) Co-IP analysis showing the inhibition of TRIF–TRAF3 and TRAF3–TBK1 interactions by TcpAh in HEK293T cells transfected with plasmids in indicated combinations.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Microbiol