FIGURE

Figure 2

ID

ZDB-FIG-210727-37

Publication

Okuda et al., 2021 - 3,4-Difluorobenzocurcumin Inhibits Vegfc-Vegfr3-Erk Signalling to Block Developmental Lymphangiogenesis in Zebrafish

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Figure 2

3,4-Difluorobenzocurcumin treatment inhibits lymphatic migration. (A) Schematic representation of the treatment schedule for larvae in images (B?E?). (B?E?) Lateral confocal images of 3 (1 days post-treatment (dpt), B,B?,D,D?) and 4 (2 dpt, C,C?,E,E?) dpf Tg(fli1a:nEGFP);Tg(-5.2lyve1b:DsRed2) larvae treated from 2.5 dpf with either 0.1% DMSO (B?C?) or 2.5 ?M 3,4-Difluorobenzocurcumin (CDF, D-E?). LEC migration is stalled in larvae treated with CDF. Images (B??E?) represent the Tg(-5.2lyve1b:DsRed2) expression of images (B?E). (F?H) Quantification of parachordal LECs (PL, F), thoracic duct (TD, G) or dorsal longitudinal lymphatic vessel (DLLV, H) nuclei per somite in Tg(fli1a:nEGFP);Tg(-5.2lyve1b:DsRed2) larvae treated with either 0.1% DMSO or 2.5 ?M CDF at indicated timepoints (n = 20 embryos/larvae). Statistical test: Kruskal-Wallis test was conducted for graph F and Mann-Whitney test were conducted for graphs G and H. p ? 0.001 (***), p ? 0.05 (*), n.s. indicates not significant. Scale bars: 100 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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