Figure 9—figure supplement 1.

(A–F) Lateral DIC images of 24hpf (A, B) or 48hpf (C–F) hai1a^hi2217 embryos treated with either DMSO (A, C, E), U0126 (B), or CI-1040 (D, F) showing rescue of general morphology (B), trunk (D), and tail (F) epidermal phenotypes compared to DMSO-treated hai1a^hi2217. (G, H) Lateral DIC images of tails of 48hpf WT treated with 125 ng/ml phorbol 12-myristate 13-acetate (PMA) alone (G) or PMA and U0126 (H). (I, J) Lateral projected confocal images of trunks of 48hpf hai1a^hi2217 embryos treated with DMSO (I) or U0126 (J) and then fluorescently immunostained for TP63. (K–M′) Lateral DIC images of the trunks (K, L, M) and tails (K′, L′, M′) of 48hpf hai1a^hi2217 embryos either untreated (M, M′), treated with 100 µM U0126 from 16hpf to 48hpf (L, L′), or treated with U0126 from 26hpf to 48hpf (M, M′). Whilst treatment from 16hpf led to a complete rescue, initiating treatment later resulted in only partial rescue. Scale bars: (B) = 200 µm; (C, H, I, M′) = 100 µm.