FIGURE 2

Generation of newborn neurons in the injured medaka is limited compared with zebrafish. (A) Schematic drawing of bromodeoxyuridine (BrdU)-labeling and stab injury in the right hemisphere of the optic tectum. Representative images of newborn neurons (BrdU⁺HuC⁺ cells) in both injured zebrafish (B) and medaka (C). White arrowheads indicate BrdU⁺HuC⁺ cells, and dashed lines indicate the injured areas. Scale bar: 50 μm. (D) Quantification of BrdU⁺HuC⁺ cells in the uninjured and injured hemispheres in zebrafish (n = 4) and medaka (n = 4). (E) Quantification of total BrdU⁺ cells in both uninjured and injured zebrafish (n = 4) and medaka (n = 4). Statistical analyses were evaluated using one-way analysis of variance followed by Tukey’s post hoc test. Statistical significance was defined as *P < 0.05.