FIGURE

Fig. 5

ID

ZDB-FIG-210716-80

Publication

Bernabé-García et al., 2021 - Telomerase reverse transcriptase (TERT) activates transcription of miR500A to inhibit Hedgehog signaling and promote cell invasiveness

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Fig. 5

Telomerase activity is not involved in miR500A up‐regulation by TERT. (A–D) Transfection of pBABE‐SAOS 2 cells with TERT or DN‐TERT (protein levels showed and quantified in (A)) to determine whether telomerase activity is necessary for miR500A promoter activity (B), for TERT‐dependent miR500A expression (C) and for the in vivo invasive capacity (D). (E, F) Inhibition by specific chemicals of TERC and TERT subunits in hTERT‐SAOS 2 cell line and its effect on the levels of miR500A (E) and on the in vivo invasive capacity (F). Each bar represents the mean ± SEM from triplicate samples, and graphs are representative of two (N = 2) (A) or three (N = 3) (B, C, E) independent experiments. Histograms represent the accumulated value of invasion percentage of a total of larvae stated in the figure for each treatment. ND, not determined; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001 according to ANOVA followed by Dunnett's (A), Tukey's (B, C, E), multiple comparison test and Fisher's exact test (D, F)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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