FIGURE 1

Survival and neutrophil counts in conventionally raised (CONV-R) and germ-free Tg(Bacmpx:GFP)ⁱ¹¹⁴ larvae inoculated with yeasts and/or Vibrio anguillarum. (A) Survival rate of germ-free and conventionally raised larvae (CONV-R) (Kaplan–Meier curves). Statistically significant differences were determined with a log-rank test (^∗p < 0.05). (B) Representation of zebrafish larvae with part of the gastrointestinal tract highlighted in red. Neutrophil counts were performed in the green area. (C) Neutrophil counts in germ-free and conventionally raised Tg(Bacmpx:GFP)ⁱ¹¹⁴ zebrafish larvae. Data show box and whisker plot: each box represents the first to third quartiles, center bar the median, and whiskers the maximum and minimum of each dataset. Different letters denote p < 0.05, Kruskal–Wallis followed by Dunn’s test. Control group: non-inoculated larvae. Dh97 and Yl242 groups: larvae inoculated by immersion with 5 × 10⁶ CFU/mL of D. hansenii 97 or Y. lipolytica 242, at 4 dpf. Va group: larvae challenged at 5 dpf by immersion with 10⁷ CFU/mL of V. anguillarum. Dh97 + Va and Yl242 + Va groups: larvae inoculated by immersion with the respective yeast at 4 dpf and challenged with V. anguillarum at 5 dpf. Data represent three independent experiments.