Gene expression during hiPSCs-to-RPE differentiation.a Bright field microscopy images (10× and 20×) of hiPSCs before (Day 0) and during their differentiation towards RPE. Note the progressive acquisition of epithelial morphology and pigmentation (observed in three independent experiments). b Hierarchical clustered heatmap showing gene expression level variations, as determined by RT-qPCR during the differentiation towards RPE from hiPSCs. Note the conservation of the two clusters (early and late transcriptional waves) identified in zebrafish.
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