Twenty-four hours postfertilization anatomical measurements. (a) Larvae exposed at 24 hpf did not display any differences in eye diameter after 1 week of recovery. (b) However, larvae in the low BPA treatment group were significantly larger than vehicle controls and larvae exposed to high BPA (p < 0.001) at 1 week post-exposure. (c) After 2 weeks, larvae exposed at 24 hpf still displayed no differences in eye diameter, despite the larger values in the high BPA treatment group. (d) Larvae exposed to high BPA had the smallest mean length at 2 weeks post-exposure. Significant differences were noted across treatments (p = 0.006), however these values were not different from the vehicle control. At each recovery point n = 5 for all treatment groups. Differences in notochord length and eye diameter across treatment were assessed using a one-way ANOVA followed by a Tukey post hoc test at each exposure age. Eye diameter measurements were normalized to notochord lengths prior to statistical analysis. All values are reported as mean ± SE. Different letters denote significance.
|
