FIGURE

FIGURE 2

ID

ZDB-FIG-210518-13

Publication

Cheng et al., 2021 - Unbiased Label-Free Quantitative Proteomics of Cells Expressing Amyotrophic Lateral Sclerosis (ALS) Mutations in CCNF Reveals Activation of the Apoptosis Pathway: A Workflow to Screen Pathogenic Gene Mutations

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FIGURE 2

Label-free quantitative proteomics of HEK293 cells transfected with different cyclin F mutations (wild type, K97R, S195R, S509P, R574Q, S621G). (A) Hierarchical group clustering of proteome profiles from transfected cells showed ALS-specific and non-ALS mutations grouped together. Immunoblot analysis (B) and LFQ proteomics (C) of relatively equal cyclin F expression levels in transiently transfected HEK293 cells. (D) Two-dimensional principle component analysis (PCA) reflects the data quality and similarities of the proteome profiles between each biological replicate that are grouped by cyclin F mutations. Figures (A,C,D) were generated using Proteome Discoverer 2.3 (Thermo).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.