FIGURE

Fig. 2

ID

ZDB-FIG-210512-84

Publication

Prajsnar et al., 2020 - The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Generation of a lyz:RFP-GFP-Lc3 transgenic line in zebrafish confirms the Lc3-mediated response to S. aureus within neutrophils. (A) Schematic of the pDEST(lyz:RFP-GFP-Lc3) construct encoding the fusion RFP-GFP-Lc3 protein under the neutrophil-specific lyz promoter. In addition, the heart marker myl7-driven GFP is used to facilitate the screening of positive larvae. (B) Confocal images at maximum projection of the Lc3-mediated response at 1 hpi in live lyz:RFP-GFP-Lc3 embryos infected with approximately 1500 CFU of mCherry-labeled S. aureus. Lyz-positive neutrophils are seen containing bacteria with (N+) or without (N-) Lc3 aggregates. Lyz-negative macrophages are also seen containing bacteria (M). The images shown are representative of three independent experiments. Arrows indicate spacious Lc3-positive vesicles, whereas arrowheads show tightly wrapped Lc3-associated bacteria. Scale bars: 10 µm. (C) Quantification of Lc3 associations with intracellular S. aureus within infected neutrophils of live lyz:RFP-GFP-Lc3 embryos at 1 hpi with approximately 1500 CFU. Data are shown as mean ± standard deviation (SD) obtained from three independent experiments (6 larvae per experiment). 79 infected neutrophils were analyzed in 18 larvae

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Autophagy