FIGURE

Figure 2

ID

ZDB-FIG-210508-5

Publication

DeLaurier et al., 2021 - ZebraShare: a new venue for rapid dissemination of zebrafish mutant data

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Figure 2

Example of how to determine DNA coordinates for a simple deletion allele, <italic>phf21aa</italic><sup xmlns:xlink='http://www.w3.org/1999/xlink'><italic>aik</italic>4</sup>.

(A) Align the WT and mutant sequence. (B) Blast the aligned WT sequence and determine the base numbers altered in the mutant. (C) Transfer these coordinates to the ZebraShare submission workbook. Descriptions become more complex for combined insertion/deletions alleles (InDels) and for alleles with multiple mutation sites due to use of multiple guide RNAs.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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