FIGURE

FIGURE 4

ID

ZDB-FIG-210506-5

Publication

Skaftnesmo et al., 2021 - Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar)

Other Figures

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FIGURE 4

Germ cell apoptosis and proliferation in WT and stra8 crispant salmon testis tissue. (A,B) Detection (A) and quantification (B) of germ cell apoptosis/DNA damage by TUNEL analysis. TUNEL + cells/cysts are shown in green and DAPI counterstain in blue. Representative germ cell stages are labeled: type A spermatogonia (SPG A), type B spermatogonia (SPG B), spermatocytes (SPC), spermatids (SPT) and spermatozoa (SPZ). Scale bar, 30 μm. Quantification results are shown as mean ± SEM (N = 3–5; *p < 0.05). (C,D) Detection (C) and quantification (D) of cell proliferation by pH3 analysis. White and black arrowheads indicate pH3 + type A undifferentiated and Sertoli cells, respectively (examples of proliferating cells are shown at higher magnification in Supplementary Figure 4C). Scale bar, 50 μm. WT, wild-type; Crispant, stra8 crispant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol