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Fig. 3

ID

ZDB-FIG-210506-14

Publication

Chen et al., 2021 - Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish

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Fig. 3

Effect of TBBPA and TiO₂ NP on larval light/dark preference and shoaling behaviors. Zebrafish embryos were exposed to 0.1% DMSO control, 2 μM TBBPA, 0.1 mg/L TiO₂ NP and TBPPA/TiO₂ NP mixture from 8 to 120 hpf and then washed three times before raising in clean fish water under standard care procedures. (A-B) Larvae at 10 dpf were used to assess light/dark background preference test (n = 24). (C-D) Larvae at 11 dpf were used to assess shoaling behaviors (n = 20). NND: nearest neighbor distance; IID: inter-individual distance. Values plotted are mean ± SEM and bars sharing the same letter indicate no significant difference at the level of P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test.

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