Figure 6

(A) Histological TRAP activity assay highlights resorption activity along the central area of the scale after 3 (T3), 4 (T4), and 5 (T5) weeks of treatment, whereas no activity is found in the scales of untreated fish (CTR), T1 (not shown) and T2 (not shown) (scale bar: 0.1 mm). (B) The percentage of scale with detectable TRAP activity staining is 50%, 70% and 80% respectively at T3, T4 and T5 (CTR vs. T3, p < 0.001; CTR vs. T4, p < 0.001 CTR vs. T5, p < 0.001). The value of the control at each experimental point was omitted as it was identical to the initial CTR. (C) Biochemical TRAP activity assay indicates a significant resorption activity in the center of scales after 4 and 5 weeks (T4 and T5) (CTR vs. T4, p < 0.01; CTR vs. T5, p < 0.001). The value of the control at each experimental point was omitted as it was identical to the initial CTR. Significance values were set at less than p < 0.01 (**), and p < 0.001 (***).