FIGURE 5

Effects of repetitive blue light stimulus exposure times and frequencies on fatigue of fictive locomotor bursting activity. Ten s‐long blue light stimuli were delivered to spinalized Tg(vglut2a:Gal4ff)^nns20;Tg(UAS:ChR2(H134R)‐mCherry)^umn201 preparations with inter‐stimulus periods of 1 (60 stimuli over 59 min), 3 (20 stimuli over 57 min), and 15 min (5 stimuli over 60 min). (a) Representative peripheral nerve recordings of fictive swimming activity produced during the first (t = 0) and final 10 s blue light stimulus delivered with each inter‐stimulus period (blue lines represent continuous 10 s blue light stimuli). (b) Number of bursts (normalized to the first stimulus at t = 0) produced during 10 s blue light stimuli delivered with each inter‐stimulus period. Red circles indicate the fifth stimulus at each inter‐stimulus period. (c) Number of bursts (normalized to the first stimulus at t = 0) produced during the fifth blue light stimulus (red circles in b) for each inter‐stimulus period (expressed as time of fifth stimulus delivery). (d) Number of bursts (normalized to the first stimulus at t = 0) produced during the final blue light stimulus, expressed as the total amount of time stimulated with blue light for each inter‐stimulus period. Shaded regions and error bars represent SD. Asterisks indicate statistically significant differences from the first stimulus. Asterisks over horizontal lines indicate significant differences between groups.