mCH accumulates in the developing nervous system. (A) mCAC/CAC levels at all CAC trinucleotides and top methylated CAC motifs in larval (W = weeks old) and adult (Ad) brains, as determined by RRBS. Data is represented as the average of two biological replicates with error bars (standard deviation). (B) RPKM (reads per kilobase per million) values of dnmt, tet, and mecp2 transcripts in larval and adult brains determined by RNA-seq. Data is represented as the average of two biological replicates with error bars (standard deviation). (C) Transcription intensities of a merged collection of differentially expressed genes called between all pairwise comparisons of larval and adult stages (r1—replicate 1, r2 = replicate 2). (D) Gene ontology enrichment of differentially expressed genes in larval and adult brains. (E) mCAC levels and relative RNA expression levels (log2 fold change 4W/Ad) at differentially expressed genes. (F) Comparisons of gene length (left), gene body mCAC/CAC (middle), and gene body mCAC/CAC at top methylated motifs (right) in genes that are either upregulated or downregulated in the adult brain (Wilcoxon test, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).
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