FIGURE

Fig. 4

ID

ZDB-FIG-210322-22

Publication

Leijten et al., 2021 - Thermal Proteome Profiling in Zebrafish Reveals Effects of Napabucasin on Retinoic Acid Metabolism

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Fig. 4

Activation and conservation of human ALDHs. The ALDH assay measures the conversion of NAD+ to NADH using a colorimetric probe (A). ALDH enzymatic activity was determined in lysates of HepG2 cells. Two concentrations of napabucasin were used (50 ?M and 100 ?M) as well as a known ALDH activator, ALDA-1 (20 ?M). 1% DMSO was used as a control, and experiments were performed in triplicate. p??? <0.001, p???? <0.0001. Significance was determined by one-way ANOVA (B). The shifting (underlined) zebrafish aldh1a2, aldh1a3, and aldh2.1 show a large degree of conservation with their human counterparts (shown in italic and bold), but show no large conservation with other zebrafish or human Aldh enzymes (C). Aldhs, aldehyde dehydrogenases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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