FIGURE

Fig. 3

ID

ZDB-FIG-210316-35

Publication

Dalgin et al., 2020 - Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b

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Fig. 3

Hoxb5b morphants form endocrine pancreas cell types but fail to form liver cells. (A–C) Control embryos; (D–F) Hoxb5b morphants. (A and D) Confocal images of representative 30 hpf Tg (sox17:EGFP) embryos. Whole mount immunolabeling for GFP (green), Islet1 (red) and Myosin to label somites (blue) of control and Hoxb5b morphant embryos. (B and E) In situ hybridization for glucagon (gcga, blue); (C and F) double in situ hybridization for ceruloplasmin (cp, blue) and insulin (ins, magenta) for control and Hoxb5b morphant embryos at 30 hpf. (C′ and F′) high magnification views of foregut region. Anterior to the left, pancreatic cells (white arrowhead) and liver cells (black arrowhead). Results are from 2 independent experiments with a minimum of 25 embryos per group. Scale bar = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 471, Dalgin, G., Prince, V.E., Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b, 1-9, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.