Fig. 6

A SorLA silencing decreases cell viability in basal and Hrg β-1-enriched cell culture conditions. Control and SorLA-silenced BT-474 cells were stimulated or not with 20 ng.mL⁻¹ Hrg β-1 for 48 h and cell viability was assessed using a WST-8-based method. Cell viability values are represented as fold change relative to unstimulated control BT-474 cells. A representative western blot validating SorLA silencing is shown. B Hrg β-1-expressing BT-474 cells were silenced for SorLA and cell viability was analyzed as in (A). A representative western blot validating SorLA silencing is shown. C, D SorLA silencing inhibits spheroid growth in Hrg β-1-enriched extracellular matrix. SorLA-silenced mCherry BT-474 cells were cocultured with Hrg β-1-overexpressing fibroblasts (Hrg β-1 TIFF) in matrigel for 12 days. Shown are representative live-cell images (C) and quantification of mCherry fluorescence reflecting spheroid growth (D). Data are mean ± SD from three independent biological replicates; statistical analysis: (A and D), One-way ANOVA, Dunn’s multiple comparisons test and (B), Student’s t test (unpaired, two-tailed, unequal variance). Scr = control non-targeting siRNA.