Figure 1

Pez Is Required for Macrophage Migration to Epithelial Wounds and Functions within the H₂O₂-Src42A-Draper Signaling Pathway

(A) Pez locus highlighting mutant alleles. Approximate CB insertion (6.056 kb) site is indicated. Pez² deletion is marked below, adapted from Poernbacher et al.¹⁷

(B) Live imaging of inflammation following laser ablation reveals reduced macrophage recruitment in Pez^CB mutants. Wound margin is denoted by dashed red line. Cell tracks are shown at 1 h.

(C) Quantification reveals a significant decrease in macrophage numbers at wounds in the two Pez mutant lines at 40 and 60 min post-injury (n ≥ 10 wounded embryos/genotype; multiple t tests with Holm-Sidak multiple comparisons).

(D and E) Cell tracking reveals (D) macrophage speed post-wounding is unaffected in Pez^CB mutants (n ≥ 130 cells from ≥5 embryos/genotype; Mann-Whitney U test), and (E) meandering index is significantly reduced in responding (cells that reach the wound site at any point within 2 h) Pez^CB macrophages (n = 53 responders from ≥5 embryos/genotype; Mann-Whitney U test).

(F) Heterozygote (src42A^[E1]/+, draper^Δ5/+, and Pez^CB/+) and transheterozygote (src42A^[E1]/Pez^CB and Pez^CB/+; draper^Δ5/+) mutant embryos at 60 min post-wounding. Wound margin is denoted by dashed red line.

(G) Significantly reduced macrophage wound recruitment in transheterozygotes embryos versus Pez^CB/+ (n ≥ 15 wounded embryos/genotype; one-way ANOVA with multiple comparisons).

All error bars are mean ± SD. NS, not significant; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005, and ^∗∗∗∗p < 0.001. All scale bars represent 20 μm. See also Figure S2 and Videos S1, S2, and S3.