Fig. 6

a–c Representative graphs of flow cytometry analysis of the TME of SW480, SW620, and MIX mouse (Rag1^−/−C57BL/6J) xenografts at 3 weeks post inoculation. d Quantification of double positive F4/80, CD80 macrophage in each TME, SW620 vs SW480. *P = 0.016, Cohen’s D g = 6.2; SW620 vs MIX ns = 0.29, Cohen’s D g = 0.83; SW480 vs MIX *P = 0.029, Cohen’s D g = 1.28). e Quantification of the percentage of each clone in MIX mice xenografts, *P = 0.029, Cohen’s D g = 7.68. d, e Data from quantification of flow cytometry analysis. Error bars represent mean ± S.D. f Growth curves of SW480 tumors treated with PBS, L-PBS, or L-Clodronate mice (f ns = 0.43, Cohen’s D g = 0.12, **P = 0.007, Cohen’s D g = 2.16, *P = 0.017, Cohen’s D g = 1.92). Error bars represent mean ± SEM. All data were analyzed using unpaired two-sided Mann–Whitney test. To avoid macrophage repopulation, mice were injected every 4 days (see “Methods”). N is depicted in the chart. Each dot represents one mouse xenograft. Source data are provided as a Source data file.