Fig 3

(A,B) qRT-PCR analysis in zebrafish PAC-2 (blue traces) and cavefish EPA (orange traces) cells after 300 μM H₂O₂ treatment. Relative mRNA expression for ddb2 (panel A) and 6–4 photolyase (panel B) are plotted on the y-axes as mean (n = 3) ± s.d.. Times (h and min) are plotted on the x-axes. Two-way ANOVA followed by Sidak’s multiple comparisons test results are reported in S1 Table. (C) qRT-PCR analysis in zebrafish and cavefish cells treated with 6 mM N-acetylcysteine (NAC) or vehicle (Ctrl), following 3 or 6 hours exposure to blue light. On the y-axes are plotted the fold induction (± s.d.) of expression with respect to samples subjected to identical treatment but maintained under DD. Times(hrs) are indicated on the x axes. Statistical analysis results (Student’s t-test (unpaired, two tailed)) are represented by asterisks (***p<0.001, **p<0.01, *p<0.05) and reported in S1 Table. (D) Representative real-time bioluminescence assays from zebrafish PAC-2 (blue trace, left side) and cavefish EPA (orange trace, right side) cells transfected with the E2F/D-box_ddb2-Luc reporter (above) and treated in DD with 300 μM H₂O₂ at the time points indicated by the blue arrows. Grey traces represent control transfected PAC-2 and EPA cells that were not treated with H₂O₂. Bioluminescence, counts per second (CPS), is plotted against time (hrs). Each time-point represents the mean of n = 8 ± s.d. Each experiment was performed a minimum of three times.