Figure 3

Raman spectroscopy reveals molecular fingerprint of FSGS serum and changes in podocyte metabolome induced by FSGS serum. (A) Mean Raman spectra of < 50 kDa serum fractions of FSGS at the time of disease recurrence (red), at the time of remission (purple) as well as of < 50 kDa serum fractions of four healthy control person (dark blue, green, brown and light blue). Different Raman signal corresponding mostly to lipoproteins were detected at the time of FSGS recurrence. Raman signals at the time of FSGS remission however resembled Raman signals of the CTRL serum fraction. Assignments of the Raman peaks according to the literature are given. (B) Representative bright field illumination (a,c) and heat map of Raman signal intensity (b,d) of cultured human podocytes treated with CTRL serum (a,b) and FSGS serum (c,d). Scale bar 10 µm. (e) Mean Raman spectra of three podocytes treated with FSGS serum (red line) and three podocytes treated with CTRL serum (blue line) showing increased Raman signal for FSGS treated cells.