Fig. 4

Fig. 4. Transgenic reporters reveal that the terminal her1 3′UTR is necessary and sufficient to confer transcript destabilization. Summary of reporter destabilization in transgenic embryos carrying various derivatives of the her1 3′UTR (A–E). All lines were raised to mid-segmentation stage and heat shocked for 15 ​min, then collected and processed by Venus in situ hybridization at 0, 30, 60, and 90 ​min pHS. (A) hsp70l:Venus-her1 3′UTR-SV40 pA reporter line (see Fig. 2, Fig. 6). (B) hsp70l:Venus-her1 3′UTRΔ1-362-SV40 pA reporter line (see Fig. 3). (C) hsp70l:Venus-her1 3′UTRΔ363-725-SV40 pA reporter line (see Fig. 3). (D) hsp70l:Venus-her1 3′UTRΔ1-546-SV40 pA reporter line (see Fig. 3). (E) hsp70l:Venus-her1 3′UTRΔ1-362;Δ547-725-SV40 pA reporter line (data not shown). Three independent lines were analyzed in wild-type embryos by in situ hybridization for each reporter, except for the hsp70l:Venus-her1 3′UTRΔ1-362;Δ547-725-SV40 pA reporter (E) for which two independent lines were analyzed. Each reporter exhibited comparable Venus decay across all independent lines (data not shown); see Methods for details. pHS ​= ​post-heat shock.