FIGURE

Fig. 1

ID

ZDB-FIG-210219-24

Publication

Shen et al., 2021 - Functional identification of an opsin kinase underlying inactivation of the pineal bistable opsin parapinopsin in zebrafish

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Fig. 1

Morpholino oligonucleotide (MO)-mediated knockdown of different GRKs in zebrafish larvae. a Nucleotide sequences of different GRK mRNAs in zebrafish. Blue and red underlining indicates GRK-MO1s- and GRK-MO2s-targeted sequences, respectively. The start codons are indicated by brackets. b Images showing whole bodies of 5 dpf larvae injected with control, GRK1b, GRK7a or GRK7b MO1s. Scale bar represents 1 mm. c Dorsal view of the pineal organs of 5 dpf larvae injected with control or one of the GRK MO1s. Landmarks indicate the pineal organs. Scale bar represents 100 μm. d Quantitative analyses of pineal size (c) in larvae injected with control MO or one of the GRK MO1s. The error bars indicate the standard error. Significance scores represent P values determined with the Wilcoxon rank sum test (NS, nonsignificant, P > 0.05)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-grk1b WT + MO1-grk7a WT + MO1-grk7b
Knockdown Reagents:	MO1-grk1b MO1-grk7a MO1-grk7b
Observed In:	epiphysis
Stage:	Day 5

Phenotype Detail

Acknowledgments

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