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Das et al., 2020 - Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish

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Fig. 1

Fig. 1. Calcium imaging in zebrafish embryos. The developing trunk muscle (DTM) of 48 h post-fertilization (hpf) zebrafish embryos were used to test the rapid effects of cortisol on Ca²⁺ dynamics using the FURA-2AM method. The treatments included cortisol antibody to sequester cortisol, EGTA to chelate extracellular Ca²⁺ (eCa²⁺), BAPTA-AM (BAPTA) to chelate intracellular Ca²⁺ (iCa²⁺), and RU486 as a GR antagonist. Also, Ca²⁺ channel blockers, including nifedipine (Nife) to block L-type channel, verapamil (VM) to block T-type channel, cadmium as a non-specific blocker of plasma membrane Ca²⁺ channels, and CpD5J-4 to block the CRAC channels were utilized. See materials and methods for details.

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Reprinted from Molecular and Cellular Endocrinology, 520, Das, C., Faught, E., Vijayan, M.M., Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish, 111067, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.