Fig. 5

Figure 5. Whole-Brain Activity Mapping Confirms the Functional Significance of Anatomically Defined Target Regions (A) Z section showing activated neurons in the PON following optogenetic stimulation in a Tg(th2:GAL4; uas:chr2-eyfp)+ fish. Magenta, tERK; green, pERK. (B) Z section showing activated neurons in the torus semicircularis following optogenetic stimulation in a Tg(th2:GAL4; uas:chr2-eyfp)+ fish. Magenta, tERK; green, pERK. (C) Z section showing activated neurons in the nMLF following optogenetic stimulation in a Tg(th2:GAL4; uas:chr2-eyfp)+ fish. Magenta, tERK; green, pERK. (D) Z section showing activated neurons in RoL-R1 following optogenetic stimulation in a Tg(th2:GAL4; uas:chr2-eyfp)+ fish. Magenta, tERK; green, pERK. (E) Z section showing activated neurons in Hc following optogenetic stimulation in a Tg(th2:GAL4; uas:chr2-eyfp)+ fish. Magenta, tERK; green, pERK. (F) Mean projection of significant, voxelwise Mann-Whitney U-statistic Z scores of an example MAP-mapping experiment. Green regions indicate increased activity following stimulation of th2+ neurons; purple regions indicate decreased activity (n = 15 fish per condition). (G) Mean projection of z-brain annotated regions showing average signal in each annotated anatomical region of the z-brain for the experiment in (F). (H) Quantified pERK ratio in regions of interest (ROIs) showing significantly increased pERK signal after stimulation of th2+ neurons. Dots are experimental replicates. Stars indicate p < 0.05; n = 6 experiments; one-tailed Wilcoxon rank sum test.