Fig. 3

CreLite‐mediated recombination at different times in development. CreLite mRNAs injected ubi:zebrabow embryos were exposed to 660 nm red light in the LED lightbox, D, at different time points (ie, 6 hpf, 1 dpf), B,C. Embryos exposed at 6 hpf and 1 dpf were assessed at 2 dpf, while those exposed at 2 dpf were assessed at 3 dpf. Mean number of embryos containing cells that exhibit yellow fluorescent protein (YFP) and/or cyan fluorescent protein (CFP) fluorescence after red light exposure were counted, E. Data are from three independent experiments and error bars represent SD; ****P < .0001, **P < .01. Ordinary one‐way analysis of variance (ANOVA) with Dunnett's multiple comparisons test. n = total number of embryos in each group. Each data point represents a clutch of embryos. Data from the negative controls (NIC, phycocyanobilin [PCB], and −660 nm) was pooled from experiments from the different time points. NIC, noninjected control. Scale bar = 1 mm