FIGURE

Fig. 3

ID

ZDB-FIG-201210-9

Publication

Everson et al., 2020 - Multifactorial Genetic and Environmental Hedgehog Pathway Disruption Sensitizes Embryos to Alcohol-Induced Craniofacial Defects

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Fig. 3

Mutations in shha sensitize embryos to multifactorial interactions between ethanol and PBO. (A) Wild‐type (+/+) or heterozygous (+/‐) embryos for shha (tq252) were exposed to subthreshold doses of PBO (3.125 μM), ethanol (0.5%), or the combination of both chemicals (3.125 μM PBO + 0.5% ethanol). Percent malformations (mild, moderate, and severe) are shown. Incidence of malformations was compared between genotypes for each treatment group using Fisher’s exact test with Bonferroni correction for multiple comparisons. (B) Quantification of inter‐trabecular widths. Mean width ± SEM is shown. Measurements were normalized to control and compared using one‐way ANOVA with Tukey’s multiple comparisons test. Different letters indicate statistically significant differences between the groups. See supplemental data for the specific multiple comparisons p‐values. N ≥ 14 embryos per genotype per treatment.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB shha^tq252/+ (AB)
Conditions:	chemical treatment by environment: ethanol chemical treatment by environment: piperonyl butoxide chemical treatment by environment: ethanol
Observed In:	neurocranial trabecula neurocranial trabecula chondrocyte
Stage:	Day 5

Phenotype Detail

Acknowledgments

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