Fig. 2.

T distributions for stimulated and control larvae at 3 and 5 dpf and spectral identity. Normalized distributions of T values for L₁, L₂, L₃, and L₄ stimulation (magenta, green, blue, and red traces, respectively; see legend) and control acquired in the absence of visual stimulation (black trace) measured for 3-dpf larvae (a) and 5-dpf larvae (b). N = 7 larvae for stimulated data; N = 5 larvae for control data in both panels; it should be noted that statistics reported in the figure are cumulated over the total number of ROIs segmented over all larvae for each condition: 847,068 (3 dpf stimulated); 588,198 (3 dpf control); 845,070 (5 dpf stimulated); 518,409 (5 dpf control). c Example traces of neurons with spectrally selective responses under standard (top panels) and shuffled (bottom panels) protocol of visual stimulation. The left panels show ΔF/F₀ traces of three neurons with a selective L₁ response; the right ones show three neurons with selective L₄ responses. Stimulus time points are shown at the top (thicker lines highlight the stimulus eliciting the selective response)