FIGURE

Figure 5—figure supplement 1.

ID

ZDB-FIG-201125-14

Publication

Nerli et al., 2020 - Asymmetric neurogenic commitment of retinal progenitors involves Notch through the endocytic pathway

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Figure 5—figure supplement 1.

Sara-positive endosomes are asymmetrically distributed during cell division.

(A) Ath5+ cells (grey) in the retinal neuroepithelium at 36 hpf in embryos injected with control-MO (upper row) and 2.5 ng/embryo Sara-MO (lower row). Scale bar, 50 μm. The yellow lines delimit the apical and basal side of the retinal neuroepithelium. (B) pH3 staining (cyan) in 36 hpf Tg(ath5:gap-GFP) embryos (grey) injected with control-MO (upper row) and 2.5 ng/embryo Sara-MO (lower row). Scale bar, 50 μm. The yellow lines delimit the apical side of the retinal neuroepithelium. (C) EdU+ cells (grey) at 36 hpf in control (upper row) and upon Sara knockdown (lower row) in Tg(ath5:gap-GFP) embryos (green). Scale bar, 50 μm. (D) Quantification of EdU-positive cells in the central portion of the retina in control (black) and Sara knockdown (blue). Unpaired t-test with Welch’s correction. Error bars represent standard deviation. (E) Example of an asymmetric inheritance of Sara-positive endosomes (left) within asymmetric division of multipotent progenitors generating Ath5+ progenitors (right). hsp70:mkate2-ras (cells membrane, grey), CFP-Sara (Sara-positive endosomes, cyan), ath5:GFP-CAAX (Ath5, green). Dashed lines show apical and basal sides of the retinal neuroepithelium. Scale bar, 10 μm. Magenta and yellow dots label sister cells. White arrow points to the Sara-positive endosome.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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