Myelin sheaths of zebrafish at 6 and 7 d postfertilization (dpf) following exposure to domoic acid (DomA) at different developmental days. Tg(mbp:EGFP-CAAX) fish were exposed to DomA over discrete developmental periods (1, 2, and 4 dpf), then imaged at (A) 6 dpf and (B) 7 dpf using widefield epifluorescence microscopy. Images were blindly classified into six categories based on the severity of the observed myelin phenotype. The scoring was as described in detail in Figure S2. Briefly, the classification was as follows: (0) normal phenotype, (1) myelin sheaths present but disorganized, (2) myelin with noticeable deficits, (3) myelin gaps in ventral spinal cord, (4) myelin sheaths lacking in ventral spinal cord, and (5) visible sloughed myelin. Stacked bar plots show the distribution of the different phenotypes per each dose. Data from individual fish from multiple trials were combined to calculate the percentage distribution per phenotype observed. Tables S25 and S27 contain the number of trials and associated numbers of fish per trial for 6 dpf- (A) and 7 dpf-injected fish (B), respectively. Table S26 includes myelin phenotype classification by dose and day injected, with imaging at 6 dpf. Table S28 includes myelin phenotype classification by DomA dose and day injected, with imaging at 7 dpf. Table S29 contains the output of the multinomial logistic regression model for the influence of dose on the distribution of myelin phenotypes.
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