Figure 2

Generation of mvp knockout zebrafish line. (A) The zebrafish mvp genomic locus and Cas9/sgRNA targeting site. Deletions in Δ22,490 allele are shown as dashes. (B) In situ hybridization and qPCR results showing the absence of mvp mRNA in mvp knock out embryos. Data are mean ± SEM. ∗∗∗∗P < 0.0001. (C) Live images of control and mvp^−/− zebrafish at designated time points. Lateral view, anterior to the left. For embryos and larvae, scale bar = 200 μm. For animal from 30 dpf to 60 dpf, scale bar = 2 mm. (D) Representative Kaplan-Meier plot for mvp^−/− fish and clutchmates from one of three independent experiments. In each experiment, 60 mvp^−/− animals and 60 total siblings were followed. n.s, not significant, Mantel-Cox test. (E) Fecundity (measured by clutch size) and mating efficiency (measured by percentage of fish laid embryos) in control and mvp^−/− zebrafish. 30 mvp^−/− fish and 30 age-matched control fish out-crossed with age-matched wild-type fish.