FIGURE

Figure 6.

ID

ZDB-FIG-201119-13

Publication

Beckwith-Cohen et al., 2020 - Controlling horizontal cell-mediated lateral inhibition in transgenic zebrafish retina with chemogenetic tools

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Figure 6.

Chemogenetic manipulation of HCs alters lateral inhibition in downstream RGCs. A, Light responses were measured in HuC::GCaMP6f fish with three light stimuli, delivered at six different spot diameters. Calcium transients were recorded using fluorescence imaging. B, WT RGCs responded with maximum change in fluorescence to a 500-μm spot of light, which decreased at 1000 μm, supporting lateral inhibition. This effect was blocked by HEPES, and was unchanged by applying 10 μm FMRFamide. C, Application of FMRFamide on FaNaC retinas perturbed normal RGC center surround response, (D) as did application of PSEM^89S on PSAM- GlyR retinas. E, The LIR in various conditions shows that application of the cognate agonists to FaNaC and PSAM retina significantly disrupts lateral inhibition. Open circles represent individual data points. Open boxes represent the interquartile range with whiskers. The mean is marked by x and the median by a line.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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