FIGURE

Fig 1

ID

ZDB-FIG-201119-1

Publication

Rajan et al., 2020 - Dual function of perivascular fibroblasts in vascular stabilization in zebrafish

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Fig 1

Characterization of perivascular fibroblasts in zebrafish.

(A) Lateral view of a three somite region in nkx3.1^NTR-mCherry; kdrl:EGFP embryos at 48 hpf. Many mCherry⁺ perivascular fibroblasts (red, arrowheads) were closely associated with intersegmental vessels (ISVs) labeled by the endothelial marker kdrl:EGFP (green). n = 11 embryos. (B) Co-expression of nkx3.1^NTR-mCherry and col1a2:GFP in perivascular fibroblasts (arrowheads) at 52 hpf. The ISV is indicated by dashed lines. Graph showing proportion of perivascular fibroblasts labeled by col1a2:GFP is shown in S1A Fig. n = 17 embryos. (C) Fluorescent mRNA in situ hybridization showing expression of fibrillar collagens col1a2 (green, left) and col5a1 (green, right) in perivascular fibroblasts (arrowheads) along ISVs marked by kdrl:EGFP (red) at 48 hpf. n = 15 embryos per staining. Scale bars: (A,C) 50 μm; (B) 25 μm.

Expression Data

Genes:	col1a2 col5a1 EGFP mCherry
Fish:	la116Tg c264Tg; ca101Tg; ca103Tg c264Tg; ca101Tg; la116Tg
Anatomical Term:	intersegmental vessel fibroblast
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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