FIGURE

Fig. 2

ID

ZDB-FIG-201015-14

Publication

Hsu et al., 2020 - CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

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Fig. 2

CHD7 regulates hematopoiesis cell autonomously. (A) Chd7 deficiency does not affect phenotypic LT-HSCs. Flow cytometry of LT-HSCs (CD48−CD150+), MPPs (CD48−CD150−), and HPC-1s (CD48+CD150−) from LinnegSca1+Kit+ (LSK) bone marrow populations (Left), which is quantified in bar graph (Right) (n = 6 to 7). Mean ± SD, unpaired two-tailed t test. (B) Schematic diagram of mouse limiting dilution transplantation experiments. (C) The frequency of functional CHD7-deficient LT-HSCs increased two-fold in whole BM (Left) and in purified CD48−CD150+LSK cells (Right) when ≥1% donor contribution to Mac1+ PB was scored at 4 mo. LT-HSC frequency was calculated by ELDA (n = 7 to 14 recipients per dose). (D) Loss of CHD7 increases multilineage hematopoiesis. (Top) Functional categories for genes up-regulated in CHD7-deficient mouse LT-HSCs were enriched for hematopoietic-related functions by IPA. Clusters of individual functional gene annotations (squares) belonging to each category are labeled numerically. Replicates: 4. (E) Heatmap of representative lineage-specific genes up-regulated in CHD7-deficient CD48−CD150+LSK cells.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA