FIGURE

Fig. 2

ID

ZDB-FIG-201012-111

Publication

Pini et al., 2020 - ALX1-related frontonasal dysplasia results from defective neural crest cell development and migration

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Fig. 2

Generation of <styled-content toggle='no' style='fixed-case'>iPSC</styled-content>‐derived NCC

Schematic of the differentiation protocol timeline. Maintenance Medium (MM) = iPSC medium (StemFlex with 1× penicillin/streptomycin), NCC differentiation medium = DMEM‐F12, 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM penicillin/streptomycin, 1 mM nonessential amino acids, 110 μM 2‐mercaptoethanol, 10 ng/ml epidermal growth factor.

Images of iPSC and iPSC‐derived NCC at Days 0, 14, and passage 4 following differentiation. Scale bars: 400 μm (Day 0), 200 μm (Day 14, passage 4).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.