Cytostatic and cytotoxic effect of PCI in AML cell lines. (A) The indicated cell lines were treated for 72 h with different concentration of PCI, or DMSO alone as a control. CTG assay was used to assess the effect of the treatment on the cell viability. The results are presented as mean ± SD from four technical replicates deriving from one independent experiment for PLB985 and AML193 cell lines, and at least two independent experiments for OCI-AML5, HL60, and THP-1 cell lines. (B) Effect of PCI on AML cell line growth. Cells were treated with 50 μM of PCI or with DMSO as a control. Cell viability (upper panel) and cell death (lower panel) were determined 12, 24, 48, and 72 h after treatment using trypan blue staining. The results are presented as mean ± SEM from two independent experiments for 12 h and three independent experiments for the others time points. (C) Cell cycle time course over 48 h of PCI treatment. Cells were treated with 50 μM of PCI or with DMSO as a control. Histogram showing cell distribution in three different cell cycle phases indicated as diverse shades of gray. The results are presented as mean ± SEM from two independent experiments. (D) Induction of apoptosis upon 72 h of PCI treatment. Cells were treated with 50 μM of PCI or with DMSO as a control. Histogram showing the percentage of live, AnnV+/PI, AnnV+/PI+ and AnnV–/PI+ cells indicated as diverse shades of gray. The results are presented as mean ± SEM from three independent experiments. NT, untreated; T0, time zero; AnnV, annexin V; PI, propidium iodide. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.
|
