ZIP9 expression in the zebrafish ovary. (A) mRNA expression of ZIP9 in different stage wildtype oocytes and follicle cells (n = 4–6). (B, C) Western blot analysis of ZIP9 protein expression on the plasma membrane of different stage wildtype oocytes/eggs (B) and the plasma membrane fraction of wildtype and zip9-/- fish ovaries (C) (n = 3–4). All data represents means ± SEM. Significance was determined by one-way ANOVA with Bonferroni multiple comparison post-test. Different letters indicate significant differences between treatment groups in the post hoc test (P < 0.05). EMV early-mid vitellogenic oocytes, FG full grown oocytes, Ov ovulated eggs, GT (EMV) granulosa/theca cells from early-mid vitellogenic follicles, GT (FG) granulosa/theca cells from full grown follicles.
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