Assessment of llgl1 mutant gross morphology. (A) Diagram depicting the 10?bp deletion generated by TALENs in exon 1 of llgl1. (B) Embryos (3?dpf) from an llgl1+/?Śllgl1?/? cross categorized into three grades of severity: class 0, wild-type phenotype; class 1 (mild), subtle signs of pericardial effusion at 3?dpf; class 2, pronounced pericardial effusion with a small subset displaying smaller eyes; and class 3 (severe), pronounced pericardial effusion and/or body edema, small eyes and hearts fail to undergo cardiac looping (Movie 5). At 5?dpf, llgl1?/? embryos are undistinguishable from llgl1+/? siblings. Scale bars: 1?mm. (C) Quantification of phenotype classes from llgl1+/?Śllgl1?/? crosses comparing maternal contribution. Three clutches were used for each condition. n=109 for maternal llgl1?/? and n=160 for paternal llgl1?/?. ?2 test. (D) Adult genotyping from six pooled llgl1+/?Śllgl1?/? crosses, greater than 4?months of age. The dashed line represents the predicted Mendelian number for each genotype. n=58 for llgl1+/? and n=31 for llgl1?/?. Two-tailed binomial test. Error bars indicate s.e.m. *P<0.05.
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