Core promoter architecture. (A) Heatmap visualizing the log2 odds ratio of occurrence of core promoter motifs between genes upregulated in G1 and S/G2/M and unchanged (*P< 0.05, **P< 0.01, ***P< 0.001, Fisher's exact test). (B) Distribution of position weight matrix (PWM) match (%) to TATA-box in the region –40 to –20 bp upstream of the dominant TSS in genes upregulated in S/G2/M and G1 populations. (C) A/T pentamer relative occurrence 20 to 40 bp upstream of TSS of genes upregulated in S/G2/M (green), G1 (red) and unchanged expression (grey). Ordering of pentamers is by best-fit match to the TATA PWM incremental thresholds shown. Pentamers significantly enriched in each group relative to pentamer occurrence in genes with unchanged expression (*P< 0.05, **P< 0.01, ***P< 0.001, Fisher's exact test). (D) Consensus cluster interquantile width in genes upregulated in S/G2/M (green) and G1 (red) and with unchanged expression (grey) visualized as a histogram. (E) Promoter shape distribution per group. Classifications; sharp (at least 90% of the expression from the promoter emanating from TSSs within 10bp of one another (IQW < 10) and ≥60% of expression emanating from a single dominant TSS); Sharp with broad background (≥60% of expression emanating from a single dominant TSS, but IQW ≥ 10) and Broad (<60% of expression emanating from a single dominant TSS and IQW ≥ 10).
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