FIGURE

FIG. 3.

ID

ZDB-FIG-200905-7

Publication

Fricke et al., 2020 - Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish

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FIG. 3.

Quantification of EGFP knockdown efficiency by fluorescence-activated cell sorting analysis. Enhanced green fluorescent protein fluorescence in (A)Tg(ddx4:ddx4-EGFP), (B)Tg(Xla.Eef1a1:mlsEGFP), (C)Tg(nkx2.5:EGFP), (D)Tg(myl7:GFP) and (E)Tg(fli1:EGFP) fish was quantified 1 dpf and 2 dpf by flow cytometry of minced and trypsinized embryos. For Tg(ddx4:ddx4-EGFP), Tg(nkx2.5:EGFP) and Tg(myl7:GFP) embryos, the number of highly fluorescent cells (at least 50-fold background fluorescence) was counted. Results are from 3 independent experiments; error bars represent one standard deviation. *P < 0.05 as compared with respective controls, ***P < 0.001 as compared with respective controls. Data are represented as mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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